The comparative study of the effect of low-intensity broadband and low-intensity pulsed ultrasound on B16 мelanoma cells in vitro

Kuzmenko, O. P., Marchenko, О., Stupak, I., Gorbach, O., Volokh, K., Hlushchak, V., & Garmanchuk, L. (2024). The comparative study of the effect of low-intensity broadband and low-intensity pulsed ultrasound on B16 мelanoma cells in vitro. Biotechnologia Acta, 17(4), 34–40. https://doi.org/10.15407/biotech17.04.034

Aim. To evaluate the effect of low-intensity broadband ultrasound (UMUS) and low-intensity pulsed ultrasound (LIPUS) on B16 melanoma cells in vitro.
Methods. The research was conducted on B16 melanoma cells under two modes of low-intensity ultrasonic sounding of cells, for which an SDG 2082 X Siglent generator (USA) with an ultrasonic emitter diameter of 20 mm was used with a frequency for UMUS of 1–7 MHz, intensity of 30 mW/cm, pulse duration of 50 μs, and for LIPUS with a frequency of 1.5 MHz, intensity of 30 mW/cm, pulse duration of 200 μs. Both modes of cell sounding were carried out with threefold irradiation, once a day after which the distribution of cells by cycle phases, the level of apoptosis and proliferative activity was determined by the method of ductal cytofluorimetry.
Results. Under the influence of UMUS ultrasonic sounding, the inhibition of the proliferation of melanoma cells by 1.6 times (P < 0.05), a 2-fold decrease in the content of aneuploid cells, compared to the control, was found. However, cytostatic and proapoptotic effects were not recorded. Under the influence of LIPUS sounding, an anti-proliferative effect, an increase in the content of dead and apoptotic cells, and decreased DI-index compared to the corresponding control were found.
Conclusions. Under the action of low-intensity broadband ultrasound (UMUS) and low-intensity pulsed ultrasound (LIPUS), a cytotoxic/cytostatic and pro-apoptotic effect, and a decrease in the content of the aneuploid cell population were revealed.