The comparative study of the effect of low-intensity broadband and low-intensity pulsed ultrasound on B16 мelanoma cells in vitro
Тип публікації :
Стаття
Дата випуску :
2024
Автор(и) :
Kuzmenko, O.P.
Marchenko, O.T.
Stupak I.V.
Volokh, K.M.
Gorbach O.I.
Hlushchak, V.V.
Мова основного тексту :
Англійська
eKNUTSHIR URL :
Журнал :
Biotechnologia Acta
Том :
17
Випуск :
4
ISSN :
ISSN 2410-776X (Online)
Початкова сторінка :
34
Кінцева сторінка :
40
Цитування :
[APA 7] Kuzmenko, O., Marchenko, O., Stupak, I., Volokh, K., Gorbach, O., Hlushchak, V., & Гарманчук, Л. В. (2024). The comparative study of the effect of low-intensity broadband and low-intensity pulsed ultrasound on B16 мelanoma cells in vitro. Biotechnologia Acta, 17(4), 34–40. https://doi.org/10.15407/biotech17.04.034
[ДСТУ] The comparative study of the effect of low-intensity broadband and low-intensity pulsed ultrasound on B16 мelanoma cells in vitro / O. Kuzmenko et al. Biotechnologia Acta. 2024. Vol. 17, no. 4. P. 34—40. DOI: 10.15407/biotech17.04.034 (date of access: 25.07.2026).
Aim. To evaluate the effect of low-intensity broadband ultrasound (UMUS) and low-intensity pulsed ultrasound (LIPUS) on B16 melanoma cells in vitro.
Methods. The research was conducted on B16 melanoma cells under two modes of low-intensity ultrasonic sounding of cells, for which an SDG 2082 X Siglent generator (USA) with an ultrasonic emitter diameter of 20 mm was used with a frequency for UMUS of 1–7 MHz, intensity of 30 mW/cm, pulse duration of 50 μs, and for LIPUS with a frequency of 1.5 MHz, intensity of 30 mW/cm, pulse duration of 200 μs. Both modes of cell sounding were carried out with threefold irradiation, once a day after which the distribution of cells by cycle phases, the level of apoptosis and proliferative activity was determined by the method of ductal cytofluorimetry.
Results. Under the influence of UMUS ultrasonic sounding, the inhibition of the proliferation of melanoma cells by 1.6 times (P < 0.05), a 2-fold decrease in the content of aneuploid cells, compared to the control, was found. However, cytostatic and proapoptotic effects were not recorded. Under the influence of LIPUS sounding, an anti-proliferative effect, an increase in the content of dead and apoptotic cells, and decreased DI-index compared to the corresponding control were found.
Conclusions. Under the action of low-intensity broadband ultrasound (UMUS) and low-intensity pulsed ultrasound (LIPUS), a cytotoxic/cytostatic and pro-apoptotic effect, and a decrease in the content of the aneuploid cell population were revealed.
Methods. The research was conducted on B16 melanoma cells under two modes of low-intensity ultrasonic sounding of cells, for which an SDG 2082 X Siglent generator (USA) with an ultrasonic emitter diameter of 20 mm was used with a frequency for UMUS of 1–7 MHz, intensity of 30 mW/cm, pulse duration of 50 μs, and for LIPUS with a frequency of 1.5 MHz, intensity of 30 mW/cm, pulse duration of 200 μs. Both modes of cell sounding were carried out with threefold irradiation, once a day after which the distribution of cells by cycle phases, the level of apoptosis and proliferative activity was determined by the method of ductal cytofluorimetry.
Results. Under the influence of UMUS ultrasonic sounding, the inhibition of the proliferation of melanoma cells by 1.6 times (P < 0.05), a 2-fold decrease in the content of aneuploid cells, compared to the control, was found. However, cytostatic and proapoptotic effects were not recorded. Under the influence of LIPUS sounding, an anti-proliferative effect, an increase in the content of dead and apoptotic cells, and decreased DI-index compared to the corresponding control were found.
Conclusions. Under the action of low-intensity broadband ultrasound (UMUS) and low-intensity pulsed ultrasound (LIPUS), a cytotoxic/cytostatic and pro-apoptotic effect, and a decrease in the content of the aneuploid cell population were revealed.
Ключові слова :
low -intensity broadband ultrasound (UMUS) low-intensity pulsed ultrasound (LIPUS) cytotoxic/cytostatic and pro-apoptotic effect B16 melanoma cells широкосмуговий ультразвук низької інтенсивності (UMUS) імпульсний ультра- звук низької інтенсивності (LIPUS) цитотоксичний/цитостатичний і проапоптотичний ефект клітини меланоми B16
Галузі знань та спеціальності :
09 Біологія
Галузі науки і техніки (FOS) :
Природничі науки
Файл(и) :![Ескіз]()
Вантажиться...
Формат :
Adobe PDF
Розмір :
378.46 KB
Контрольна сума :
(MD5):8026e8bee001e408013bb81563714c65
Якщо не вказано інше, ця робота розповсюджується на умовах ліцензії Creative Commons Attribution 4.0 International