Study of the Antioxidant Activity of Synthetic 3-hydroxyflavone Derivatives by DPPH and Hydrogen Peroxide Methods

Khdera, H., Saad, S., Moustapha, A., Kandil, F. (2023). Study of the Antioxidant Activity of Synthetic 3-hydroxyflavone Derivatives by DPPH and Hydrogen Peroxide Methods. French-Ukrainian Journal of Chemistry, 11(2), 19–30. https://doi.org/10.17721/fujcV11I2P19-30

Flavonoids have good efficacy as antioxidants due to their ability to scavenge free radicals within cells. In our previous study, synthetic flavonoid derivatives (A2-A13) were prepared in vitro through condensation, oxidative cyclization, alkylation and esterification reactions. The antioxidant activity of the alkyl and esterified derivatives of the flavonoid compound (A2) 3-hydroxy-2-(4-[dimethylamino]phenyl) benzo-4-pyrone was studied by two methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition method and inhibition of hydrogen peroxide (H2O2) and compared to the activity of standard antioxidant compounds, ascorbic acid and quercetin. The flavonoids (prepared samples) A2, A5, and A7 showed high activity approaching that of ascorbic acid and quercetin as well-known antioxidants. As for the compounds (A3, A6, A8, A9, A10, A11, A12, A13), which contain an -OH enol group, a benzyl group, and a chlorine group and the acetate group, its effectiveness has decreased significantly.