Comparison of 2D and 3D primary cell cultures obtained from explant of high-grade urothelial bladder cancer
Тип публікації :
Стаття
Дата випуску :
2023
Автор(и) :
P.G. Yakovlev
Yu.A. Stupak
O.V. Skachkova
O.I. Gorbach
Yu.V. Stepanov
Мова основного тексту :
Англійська
eKNUTSHIR URL :
Журнал :
Experimental Oncology
Том :
45
Випуск :
1
ISSN :
1812-9269
Початкова сторінка :
130
Кінцева сторінка :
136
Цитування :
[APA 7] Гарманчук, Л. В., P.G., Y., Островська, Г. В., Yu.A., S., O.V., S., O.I., G., & Yu.V., S. (2023). Comparison of 2D and 3D primary cell cultures obtained from explant of high-grade urothelial bladder cancer. Experimental Oncology, 45(1), 130–136. https://doi.org/10.15407/exp-oncology.2023.01.130
[ДСТУ] Comparison of 2D and 3D primary cell cultures obtained from explant of high-grade urothelial bladder cancer / Л. В. Гарманчук et al. Experimental Oncology. 2023. Vol. 45, no. 1. P. 130—136. DOI: 10.15407/exp-oncology.2023.01.130 (date of access: 25.07.2026).
Studying the biological characteristics of bladder cancer in primary culture can be an effective way for diagnostic and prognostic purposes, as well as choosing a scheme for personalized therapy.
Aim: To characterize and compare 2D and 3D primary cell cultures obtained from the same tumor sample resected from a patient with high-grade bladder cancer.
Materials and Methods: 2D and 3D primary cell cultures were obtained from explants of resected bladder cancer. Glucose metabolism, lactate dehydrogenase (LDH) activity, and level of apoptosis were studied.
Results: Multicellular tumor spheroids (3D) differ from planar culture (2D) by more pronounced consumption of glucose from the culture medium (1.7 times higher than 2D on Day 3 of culture), increased lactate dehydrogenase activity (2.5 times higher on Day 3 vs. Day 1 of cultivation, while in 2D culture LDH activity is constant), stronger acidification of the extracellular environment (pH dropped by 1 in 3D and by 0.5 in 2D). Spheroids demonstrate enhanced resistance to apoptosis (1.4 times higher).
Conclusion: This methodological technique can be used both for tumor characterization and for selection of optimal postoperative chemotherapeutic schemes.
Aim: To characterize and compare 2D and 3D primary cell cultures obtained from the same tumor sample resected from a patient with high-grade bladder cancer.
Materials and Methods: 2D and 3D primary cell cultures were obtained from explants of resected bladder cancer. Glucose metabolism, lactate dehydrogenase (LDH) activity, and level of apoptosis were studied.
Results: Multicellular tumor spheroids (3D) differ from planar culture (2D) by more pronounced consumption of glucose from the culture medium (1.7 times higher than 2D on Day 3 of culture), increased lactate dehydrogenase activity (2.5 times higher on Day 3 vs. Day 1 of cultivation, while in 2D culture LDH activity is constant), stronger acidification of the extracellular environment (pH dropped by 1 in 3D and by 0.5 in 2D). Spheroids demonstrate enhanced resistance to apoptosis (1.4 times higher).
Conclusion: This methodological technique can be used both for tumor characterization and for selection of optimal postoperative chemotherapeutic schemes.
Галузі знань та спеціальності :
09 Біологія
Галузі науки і техніки (FOS) :
Природничі науки
Файл(и) :![Ескіз]()
Вантажиться...
Формат :
Adobe PDF
Розмір :
2.45 MB
Контрольна сума :
(MD5):d451bba493481281c9851d16fe08f1c5
Якщо не вказано інше, ця робота розповсюджується на умовах ліцензії Creative Commons Attribution 4.0 International