Effect of the Secretome of Mesenchymal Placenta Stem Cells on the Functional Properties of Lewis Lung Carcinoma Cells In Vitro

Журнал :
Cytology and Genetics
Том :
58
Випуск :
4
ISSN :
ISSN 0095-4527
Початкова сторінка :
283
Кінцева сторінка :
291
Цитування :
[APA 7] Гарманчук, Л. В., Островська, Г. В., Stupak, I. A., Bukreieva, T., Gorbach, O., & Остапченко, Л. І. (2024). Effect of the Secretome of Mesenchymal Placenta Stem Cells on the Functional Properties of Lewis Lung Carcinoma Cells In Vitro. Cytology and Genetics, 58(4), 283–291. https://doi.org/10.3103/S0095452724040054
[ДСТУ] Effect of the Secretome of Mesenchymal Placenta Stem Cells on the Functional Properties of Lewis Lung Carcinoma Cells In Vitro / Л. В. Гарманчук et al. Cytology and Genetics. 2024. Vol. 58, no. 4. P. 283—291. DOI: 10.3103/S0095452724040054 (date of access: 18.07.2026).
This paper concerns the effect produced by the components of a conditioned medium (K-medium), in which mesenchymal human placenta cells (hP-MSC) are cultivated, on the characteristics of Lewis lung carcinoma (LLC) cells in the culture. It is shown for the first time that the K-medium (secretome) components have a prooncogenic effect on LLC cells as evidence by an increase in cell survival rates, LLC cell proliferation stimulation, and a decrease in the level of apoptotic cells. The effect of the K-medium on the adhesion characteristics of LLC cells in the process of their monolayer growth and migration from 3Dcultures is also demonstrated. When the hP-MSC secretome interacts with the cultured LLC cells, the production of proinflammatory cytokines TGF β and Il-6 is observed to grow. At the same time, the proangiogenic factor VEGF remains almost at the same level. Similar changes in the microenvironment during the interaction of mesenchymal and tumor cells may underlie various prooncogenic effects observed in our previous
studies with different MSC inoculation methods during the development and metastasis of Lewis lung carcinoma in vivo.
Ключові слова :
Lewis lung carcinoma (LLC) cells mesenchymal stem cells cryopreserved human placenta conditioned medium TGF β Il-6 prooncogenic effects
Галузі знань та спеціальності :
09 Біологія
Галузі науки і техніки (FOS) :
Природничі науки
Файл(и) :
Вантажиться...
Ескіз
Формат :

Adobe PDF

Розмір :

732.71 KB

Контрольна сума :

(MD5):ae35289f594fc824e6c81e9b56750861

Creative Commons Attribution 4.0 International
Якщо не вказано інше, ця робота розповсюджується на умовах ліцензії Creative Commons Attribution 4.0 International