C60 Fullerene prevents genotoxic effects of doxorubicin in human lymphocytes in vitro

Afanasieva, K. S., Prylutska, S. V., Lozovik, A. V., Bogutska, K. I., Sivolob, A. V., Prylutskyy, Y. I., Ritter, U., & Scharff, P. (2015). C(60) fullerene prevents genotoxic effects of doxorubicin in human lymphocytes in vitro. The Ukrainian Biochemical Journal, 87(1), 91–98. https://doi.org/10.15407/ubj87.01.091

The self-ordering of C60 fullerene, doxorubicin and their mixture precipitated from aqueous solutions was investigated using atomic-force microscopy. The results suggest the complexation between the two compounds. The genotoxicity of doxorubicin in complex with C60 fullerene (С60+Dox) was evaluated in vitro with comet assay using human lymphocytes. The obtained results show that the C60 fullerene prevents the toxic effect of Dox in normal cells and, thus, С60+Dox complex might be proposed for biomedical application.